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A sample of otolith material for analysis was taken from outside a 150 μm radius from the otolith core using a micromilling technique using a MicroMill (New Wave Research Inc., USA) (Fig. 2). The otolith central portion (less than 150 μm from the core), which corresponded to the seagoing leptocephalus stage, had been previously removed by drilling to avoid contamination. Micromilled samples ...

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